The responses of earthworms to atrazine different concentrations in three kinds of forest soils were quantitatively determined by laboratory test. In the toxicity test, 40, 80, 160, 320 and 480 mg/kg were used as exposure concentrations of atrazine, and the fresh weight, body width and mortality of earthworms were measured at 0, 7, 14, 21, 28 and 35 d after exposure. The results showed that lethal effect and inhibitory effect of atrazine on the fresh weight and body width of earthworms increased with the exposure concentration and time with obvious time-effect and dose-effect relationship. At the same concentration, the effect of atrazine on earthworms in the three soils was expressed as typical yellow brown soil > weakly developed yellow brown soil > lime soil. The toxic effect of atrazine on earthworms was negatively correlated with the content of organic matter in soil, and positively correlated with the content of total phosphorus and available phosphorus. The results of the study revealed that the toxic effects of atrazine on earthworms in different types of soil differed significantly, and correlated well with soil fertility, which could provide basic reference data for assessing the environmental risks of atrazine use in agriculture and forestry.