Based on the data of topsoil available Zn contents of croplands in 1984-2020 in Yangzhou City, geostatistical analysis and correlation analysis were applied to systematically analyze spatio-temporal variation characteristics of available Zn and its influencing factors. The results showed that available Zn increased meanly by 1.15 mg/kg and by 188.5% in the past 37 years. Available Zn was spatially heterogeneous, which was high in south and west, but low in north and east. Available Zn was significantly different between different parent materials, soil textures and soil utilizations, among of which, the Yangtze River alluvial deposit had the higher available Zn than other parent materials, sandy loam soil had higher available Zn than other textures, dry field had higher available Zn than other land use types. Additionally, soil available Zn had certain relationship with soil pH and organic matter.