Soil aggregates, as the basic unit of soil, to a certain extent reflect the quality of the soil. Manure application can affect the formation and stability of soil aggregates by increasing the amount of organic matter and nutrients in soil. Most studies found that manure application increased the proportion of soil marcoaggregate (>0.25mm). However，there are significant differences among studies of the result that manure application affect large marcoaggregate (>2mm) more or small marcoaggregate (2-0.25mm). We conducted a meta-analysis based on 36 studies with 267 treatments and found that manure application significantly increased the proportion of soil large macroaggregates and small marcoaggregate by 95% and 17%, respectively. At the same time, it reduced the proportion of soil microaggregates and silt-plus and clay-size particles by 14% and 20%, respectively. What’s more, meta-regression analyze found that soil types, soil pH, manure types, annual temperature, and annual rainfall are the main factors affecting the responses of manure application to soil aggregate.