福建省典型杉木人工林根际土壤氧化亚氮还原菌丰度特征
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S154.36

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国家自然科学基金项目(31971447)、福建省自然资源科技创新项目(KY-090000-04-2022-012)和闽江学院引进人才预研项目(MJY20012)资助。


Gene Abundance of N2O Reducers in Rhizosphere Soils of Typical Chinese Fir Plantations from Fujian Province
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    摘要:

    为揭示福建省典型杉木人工林根际土壤中氧化亚氮(N2O)还原菌的丰度及其关键环境驱动因子,选取3个典型林场的杉木人工林为对象,利用实时荧光定量PCR测定了根际土壤N2O还原菌功能基因nosZ I和nosZ II丰度。结果表明:3个典型杉木人工林根际土壤的nosZ I基因丰度均显著高于nosZ II,表明nosZ I在酸性杉木人工林根际土壤中占主导,这可能与nosZ II型N2O还原菌对低pH比较敏感有关;3个林场中,武夷山国家森林公园的杉木人工林根际土壤nosZ I基因丰度为2.53×107 copies/g,显著高于三明国家野外科学观测研究站(9.27×106 copies/g)和上杭白砂国有林场(8.90×106 copies/g),但3个林场之间的nosZ II丰度差异不显著;随机森林分析表明,土壤pH是调控N2O还原菌nosZ I和nosZ II丰度的关键环境因子。综上所述,福建省不同区域典型杉木人工林根际土壤nosZ I基因丰度存在差异,土壤pH是最主要的调控因子。

    Abstract:

    In order to reveal the abundance of N2O reducers and their critical driving factors in the rhizosphere soils of typical Chinese fir plantation from Fujian Province, rhizosphere soil samples under Chinese fir plantation from three typical forest farms in Fujian Province were collected. The abundance of functional genes of soil N2O reducers, namely nosZ I and nosZ II, were determined using real-time quantitative PCR. Results showed that the abundance of nosZ I gene was significantly higher than that of nosZ II in the rhizosphere soils of Chinese fir plantation in all the three typical forest farms, which suggested that nosZ I-type N2O reducers dominate in the acidic rhizosphere soils of Chinese fir plantation, possibly due to the fact that the nosZ II-type N2O reducers are more sensitive to low pH conditions. Among the three forest farms, the abundance of nosZ I gene was 2.53×107 copies/g in Wuyishan National Park, and significantly higher than 9.27×106 copies/g in Sanming Forest Ecosystem National Observation and Research Station and 8.90×106 copies/g in Shanghang Baisha Stated-owned Forest Farm. However, there was not significant difference in nosZ II abundance among the three forest farms. Random forest analysis showed that soil pH was the key environmental factor regulating the abundance of nosZ I and nosZ II. In conclusion, the abundance of nosZ I gene varies substantially in the rhizosphere soils of Chinese fir plantation from three typical forest farms in different regions of Fujian Province, while soil pH is the most important driving factor.

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左婷,孙其荣,陈婧涵,罗培傑,吴黄垒,郑勇,林永新,叶桂萍.福建省典型杉木人工林根际土壤氧化亚氮还原菌丰度特征[J].土壤,2024,56(5):992-998. ZUO Ting, SUN Qirong, CHEN Jinghan, LUO Peijie, WU Huanglei, ZHENG Yong, LIN Yongxin, YE Guiping. Gene Abundance of N2O Reducers in Rhizosphere Soils of Typical Chinese Fir Plantations from Fujian Province[J]. Soils,2024,56(5):992-998

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  • 收稿日期:2023-11-23
  • 最后修改日期:2024-02-03
  • 录用日期:2024-02-05
  • 在线发布日期: 2024-11-14
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