In order to screen out high efficiency passivating agent of arsenic(As) in soil contaminated by heavy metals, the effects of four passivating agents, maize straw biochar (MBC), peanut shell biochar (PBC), attapulgite (ATP) and hydroxyapatite (HAP), on adsorption and desorption of As in soil were investigated by the batch test. The results showed that passivators significantly increased the adsorption amount and adsorption rate of As in soil (P<0.05) with an order of HAP>ATP>PCB>MBC>CK. Langmuir equation was better for the isothermal sorption curve (P<0.01). The four passivators increased the maximum adsorption amount and binding strength of As in soil, and the maximum adsorption amount followed the order of ATP>PBC> MBC>HAP>CK. Adsorption-desorption test indicated the adsorption kinetic curves of As could be described well by the Elovich equation (P<0.01), and HAP and ATP had better adsorption effect, but the desorption amount of HAP was 25.0%-86.3% higher than that of ATP. Therefore, from the perspective of adsorption and desorption, ATP is a better passivating agent for As in soil.