耐低温秸秆降解复合菌系的构建及功能验证
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X172

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国家重点研发计划项目(2022YFD1500202)和中国科学院战略性先导科技专项(A类)(XDA28020202)资助。


Construction and Functional Verification of Low-temperature-resistant Straw Degrading Synthetic Bacterial Consortium
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National Key R&D Program of China (2022YFD1500202) and Strategic Priority Research Program of Chinese Academy of Sciences (XDA28020202)

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    摘要:

    为了挖掘耐低温的纤维素降解菌并构建复合菌系来提高秸秆利用率,增加土壤有机质含量,本文利用羧甲基纤维素(CMC)选择性培养基和刚果红鉴别培养基从长期秸秆还田土壤中筛选能利用纤维素的菌株,并通过控温试验,测定CMC酶活以及秸秆降解率等指标挖掘菌株的低温降解潜力;同时选取分离自南极的节杆菌(Arthrobacter sphaeroides) AS-3(保藏于中国典型培养物保藏中心CCTCC)作为阳性对照,进行耐低温复合菌系的构建及玉米秸秆降解水平的测定。结果表明,单菌CN8、CN10和CN16(均为芽孢杆菌Bacillus sp.)能在低温条件下有效降解玉米秸秆,而三者构建的复合菌系D能更高效地提升玉米秸秆的降解能力,表现为15℃恒温培养30 d后对玉米秸秆的降解率能达到26%以上,超过了单菌和阳性对照菌株。由此,本研究获得的耐低温复合菌系D,具有较强的秸秆降解能力,这一结果丰富了东北黑土的有益微生物资源,具有良好的应用前景,并为低温生境秸秆资源利用提供理论基础。

    Abstract:

    In order to improve the utilization of straw and increase soil organic matter by excavating low-temperature-resistant cellulose-degrading bacteria and constructing synthetic bacterial consortium (SynCom), carboxymethyl cellulose (CMC) selective medium and congo red discriminating medium were used to screen cellulose-utilizing strains from soil under long-term straw return. Through temperature control experiments, CMC enzyme activity and straw degradation rate were measured to explore the low-temperature degradation potential of the strains. Meanwhile, Arthrobacter sphaeroides AS-3 isolated from Antarctica (deposited in the Chinese Collection of Typical Cultures, CCTCC) was selected as a positive control, and the construction of low-temperature-resistant SynCom and the determination of the degradation level of maize stover were carried out. The results showed that the single bacteria CN8, CN10 and CN16 (all are Bacillus sp.) effectively degraded corn straw at low temperature, while SynCom D constructed by the bacteria enhanced the degradation of corn straw more efficiently. The degradation rate of corn straw reached more than 26% after 30 d of incubation at a constant temperature of 15 ℃, which was more than the degradation rate of the single bacteria and the positive control strains. In conclusion, the low-temperature-resistant SynCom D has strong straw degradation ability, can enrich the beneficial microbial resources in the northeastern black soil, thus has a good application prospect and provide a theoretical basis for the utilization of straw resources in low-temperature habitats.

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赵丹,徐希媛,张玉潇,徐志辉,李琪,姚彦坡,褚海燕.耐低温秸秆降解复合菌系的构建及功能验证[J].土壤,2025,57(2):300-307. ZHAO Dan, XU Xiyuan, ZHANG Yuxiao, XU Zhihui, LI Qi, YAO Yanpo, CHU Haiyan. Construction and Functional Verification of Low-temperature-resistant Straw Degrading Synthetic Bacterial Consortium[J]. Soils,2025,57(2):300-307

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  • 收稿日期:2024-02-07
  • 最后修改日期:2024-04-01
  • 录用日期:2024-04-07
  • 在线发布日期: 2025-05-08
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