This study investigated the characteristics of iron toxicity on the yield traits of rice and explored its relationship with iron contents in different parts and in grains. The results showed that iron toxicity significantly inhibited the leaf length, plant height, tiller number and panicle number of rice, while also reduced the length and width of grains, resulting in a significant decrease in 1000-grain weight. Under iron toxicity, most of iron was sequestered in the roots. Among the various aboveground parts, iron content increased most significantly in the old leaves, with no significant increase in new leaves and mature leaves. Iron content in grains also significantly increased under iron toxicity.