基于天然腐殖质材料土壤改良的设施姜地青枯病防治研究
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S156.99

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安徽省重点研发计划项目(2023n06020056)、中国科学院战略性先导科技专项(XDA0440000)和国家重点研发计划项目(2022YFD1500903,2022YFD1500502)资助。


Study on Prevention and Control of Ginger Bacterial Wilt in Facility Cropping Through Soil Improvement with Natural Humic Materials
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Supported by the Key Research and Development Program of Anhui Province (No. 2023n06020056), Strategic Pioneering Science and Technology Special Project of Chinese Academy of Sciences (No. XDA0440000), the National Key Science and Technology Project (No. 2022YFD1500502, No. 2022YFD1500903)

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    摘要:

    通过田间试验,设置3个处理,分别为不施用有机物料(CK)、施用天然腐殖质材料(MT)、施用有机肥(OF),探究天然腐殖质材料对连作姜地土壤理化性质、细菌群落组成和生姜青枯病病情指数的影响。试验结果表明,MT处理显著改变了土壤物理结构,与CK相比,容重从1.35 g/cm3降至1.16 g/cm3,降低了14.1%;同时大团聚体含量得到显著提高,MT处理中>2 mm团聚体含量提高了56.8%,2~0.25 mm团聚体含量提高了59.6%。在扩充养分库容上,MT和OF处理均能显著提高土壤有机质,增强土壤养分有效性。同时,MT处理能显著提高土壤pH。此外,与CK处理相比,MT处理显著增产155%。土壤理化性质与病情指数相关性分析表明,土壤养分指标和2~0.25 mm水稳性团聚体与病情指数呈负相关关系。与CK处理相比,MT处理显著提高了土壤细菌群落α、β多样性,其细菌群落变化主要由全氮和0.25~0.053 mm水稳性团聚体含量驱动。细菌共现网络发现,天然腐殖质材料能够促进土壤中细菌的相互作用,提高土壤中细菌网络的模块多样性,强化细菌网络的结构和功能稳定性;生姜病情指数主要与Peribacillus_31373GaiellaAG11细菌直接相关,本试验中天然腐殖质材料处理主要通过影响Peribacillus_31373GaiellaAG11细菌进而抑制青枯病发病情况。上述结果表明:通过施用天然腐殖质材料能有效改善设施姜地物理结构变劣、养分库容减小的问题,提高生姜产量,并显著缓解生姜青枯病的发病情况,主要通过影响土壤养分指标、2~0.25 mm水稳性团聚体和土壤中重要菌属的丰度来抑制青枯病的发生。

    Abstract:

    This study conducted field experiments to investigate the impacts of natural humic materials on soil physicochemical properties of continuously cropped ginger, bacterial community composition, and the disease index of ginger bacterial wilt. Three treatments were set up: no application of organic materials (CK), natural humic materials (MT), and organic fertilizer (OF). The results showed that MT significantly altered soil physical structure, compared to CK, the bulk density decreased from 1.35 g/cm3 to 1.16 g/cm3, decreased by 14.1%. Simultaneously, the content of large aggregates (>2 mm) significantly increased by 56.8%, while the content of aggregates (0.25-2 mm) increased by 59.6%. Regarding the expansion of nutrient storage capacity, both MT and OF significantly increased soil organic matter, enhancing nutrient availability. Additionally, MT significantly increased soil pH, and it also increased ginger yield by 155% compared to CK. Disease index had a negative correlation with soil nutrients and water-stable aggregates of 2-0.25 mm. Compared to CK, MT significantly improved the α and β diversity of soil bacterial communities. The changes in bacterial communities were primarily driven by total nitrogen and the content of water-stable aggregates of 0.25-0.053 mm. Furthermore, the bacterial co-occurrence network revealed that natural humus materials could promote the interactions among soil bacteria, enhancing the module diversity and reinforcing the stability of bacterial networks. Interestingly, disease index was mainly directly related to Peribacillus_31373, Gaiella and AG11 bacteria, and natural humus materials inhibited the incidence of bacterial wilt mainly by affecting the bacteria of Peribacillus_31373, Gaiella and AG11. In conclusion, applying natural humus materials could effectively improve soil physical structure and increase soil nutrient reservoir of ginger facility, increase ginger yield, and significantly alleviate the incidence of bacterial wilt mainly by affecting soil nutrient index, water-stable aggregates of 2-0.25 mm and the abundance of important soil bacteria.

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朱良辉,张丛志,潘慧,王红叶,张佳宝.基于天然腐殖质材料土壤改良的设施姜地青枯病防治研究[J].土壤,2025,57(2):308-317. ZHU Lianghui, ZHANG Congzhi, PAN Hui, WANG Hongye, ZHANG Jiabao. Study on Prevention and Control of Ginger Bacterial Wilt in Facility Cropping Through Soil Improvement with Natural Humic Materials[J]. Soils,2025,57(2):308-317

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  • 收稿日期:2024-03-24
  • 最后修改日期:2024-04-03
  • 录用日期:2024-04-15
  • 在线发布日期: 2025-05-08
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