In order to fully understand the current status of soil nutrients in the tobacco-growing areas in southeastern Chongqing, a total of 1 421 soil samples were collected in five districts and counties in southeastern Chongqing from 2021 to 2022, and soil nutrient contents were measured and compared with those in 2012 to clarify their change trend and give a reasonable fertilization plan. The results showed that compared with 2012, soil pH increased slightly, organic matter content decreased slightly, and the contents of available phosphorus and potassium increased significantly in 2022. At present, soil with acidic pH accounted for 36.88%, soil with low organic matter accounted for 23.08%, and soil with suitable alkaline nitrogen, available phosphorus and potassium, exchangeable calcium and magnesium accounted for 74.17%, 20.97%, 11.89%, 44.19% and 30.96%, respectively. In general, soil acidification is serious, soil organic matter is relatively insufficient, alkaline nitrogen is suitable, the available phosphorus and potassium are generally high, available manganese, zinc, molybdenum, sulfur and boron are rich, while exchangeable calcium and magnesium, available iron and copper are insufficient in a large proportion, and the deficiency and excess of each element coexist. Therefore, the tobacco-growing areas in southeastern Chongqing should adopt measures according to the current status of soil nutrients and the fertilizer zoning maps, applying fertilizers scientifically and rationally to achieve the sustainability of soil and flue-cured tobacco production.