To explore the effects of selenium application on the total selenium content, inorganic and organic selenium content in wheat grains, with no selenium application as the control group, a meta-analysis was used to comprehensively analyze the effects of factors such as selenium source, selenium application season, selenium application frequency, selenium application amount, and selenium application method on wheat selenium absorption. The results showed that selenium sources can increase the total selenium content in wheat grains, with the highest effect of selenate (253%), but there was no significant difference between different selenium sources. The response of selenium uptake in wheat varies at different seasons, with better effects observed during the late-growth stage (booting stage filling stage), especially during the flowering stage (216%) and irrigation stage (217%). The maximum effect (>200%) on the inorganic and organic selenium content in wheat grains was observed when the selenium application rate was 60-100 g .hm_^-2. The promotion effect of soil application on the total selenium content in wheat grains is significantly higher than that of foliar spraying (up to 39.5%), but foliar spraying is more conducive to the accumulation of inorganic selenium in grains. Applying selenium less than or equal to 2 times has a significant promoting effect on the selenium content in wheat grains, but the promoting effect decreases as the frequency increases. In practical production, various factors can be comprehensively considered to select selenate, apply selenium in the later stage of growth, reduce the frequency of selenium application (≤ 2), and adjust the amount of selenium application appropriately (≤ 100 g .hm_^-2) to maximize the enrichment of selenium in wheat.