不同类型氮对温带森林土壤惰性有机碳库的影响
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S714;X171

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河北省自然科学基金项目(C2024208003,C2020208001)和河北省高层次人才项目(A201901041)资助。


Effects of Different Nitrogen Types on Soil Recalcitrant Organic Carbon Pool in Temperate Forests
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    摘要:

    土壤惰性有机碳(ROC)高度稳定且占比较高,是土壤有机碳库的重要组成部分,其含量变化指示土壤碳库动态。外源氮输入是影响ROC的一个关键因素,然而,不同氮类型如何影响ROC尚不明晰。基于此,本研究通过向温带森林土壤分别施加等氮量(N 10.0 g/(m2·a))的硝态氮(KNO3、NaNO3)、铵态氮(NH4Cl、(NH4)2SO4)、铵硝混合氮(NH4NO3)和酰胺态氮(Urea),研究了不同类型氮源对温带森林土壤ROC的影响。结果表明:不同类型氮添加均显著提高了土壤ROC的含量和惰性碳指数(RI),增幅分别为73.4%~161.9% 和52.3%~137.4%。其中,铵态氮处理后ROC含量增幅最大,NH4Cl和(NH4)2SO4处理增幅分别为133.7% 和161.9%,原因是铵态氮对木质素过氧化物酶、漆酶、多酚氧化酶活性的抑制和对pH的降低作用最强,其分别下降了35.7%~38.7%、24.9%~25.3%、38.1%~56.9% 和10.8%~11.4%。酰胺态氮处理对上述酶活性的抑制作用最弱,降幅分别为13.8%、14.6% 和24.6%,对ROC含量的增加也相对最低(73.4%)。硝态氮施加导致锰过氧化物酶活性降幅最大,KNO3和NaNO3处理的降幅分别为10.5% 和24.0%,进而导致ROC含量增加109.6%~111.0%。综上,在准确预测和评估ROC对氮沉降的响应时,应考虑氮源类型的影响。

    Abstract:

    Soil recalcitrant organic carbon (ROC) is a highly stable component of soil organic carbon. It accounts for a relatively high proportion of the soil organic carbon pool, and the change in its content indicates the dynamics of the soil organic carbon pool. Exogenous nitrogen (N) input is a key factor affecting soil ROC. However, how different types of N components affect ROC is still unclear. Based on this, nitrate N (KNO3 and NaNO3), ammonium N (NH4Cl and (NH4)2SO4), mixed N (NH4NO3), and amide N (Urea) were fertilized with equal amounts (N 10.0 g/(m2·a)) to temperate forest soils to investigate the effects of different N fertilizers on temperate forest soil ROC. The results showed that different types of N addition significantly increased soil ROC content and recalcitrant carbon index (RI) by 73.4%-161.9% and 52.3%-137.4%, respectively. Among them, the increase of ROC content after ammonium N treatment was the largest, and the increase of ROC content after NH4Cl and (NH4)2SO4 treatment was 133.7% and 161.9%, respectively. It was because ammonium N showed the strongest inhibition effect on the activities of lignin peroxidase, laccase, polyphenol oxidase, and the greatest reductuion effect on pH, which decreased by 35.7%-38.7%, 24.9%-25.3%, 38.1%-56.9%, and 10.8%-11.4%, respectively. Amide N showed the weakest inhibition on the activities of these enzymes (declined by 13.8%, 14.6%, and 24.6%, respectively), and the increase in ROC content was correspondingly lower (73.4%). Nitrate N addition declined most the activity of manganese peroxidase, and the decreases of KNO3 and NaNO3 treatments were 10.5%, and 24.0%, respectively, which led to an increase of ROC content by 109.6%-111.0%. In conclusion, the impacts of N types should be considered when accurately predicting and evaluating the response of ROC to N deposition.

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左甜,赵涵,苗梦雨,刁静文,张晓慧,高哲,郭鹏.不同类型氮对温带森林土壤惰性有机碳库的影响[J].土壤,2026,58(3):598-604. ZUO Tian, ZHAO Han, MIAO Mengyu, DIAO Jingwen, ZHANG Xiaohui, GAO Zhe, GUO Peng. Effects of Different Nitrogen Types on Soil Recalcitrant Organic Carbon Pool in Temperate Forests[J]. Soils,2026,58(3):598-604

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  • 收稿日期:2025-02-27
  • 最后修改日期:2025-06-17
  • 录用日期:2025-06-18
  • 在线发布日期: 2026-07-15
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