Limestone soil is an important type of soil, covering about 17.5% of the total of the soils in Guizhou. According to definition of the Chinese Soil Taxonomy and genetic classification, limestone soil must be derived from weathered bedrock carbonate rock. However, it is hard to determine origin of limestone soils only on the basis of field observation, or the lithologic characters of carbonate rock in layer cited by the soil classification. So, origin of limestone soil has long been a controversial topic. In this paper, further exploration is made by taking four limestone soil profiles as example, which are developed over bedrocks of dolomitite and limestone and located in upland in the central, north Guizhou. The coherence of mineral, Ti/Al, Al/Zr, Ti/Zr, Nb/Ti, REE and particle-size characteristics implies that there are inherited relationship among the bedrock, insoluble residues of bedrock and soil layer. It can be concluded that limestone soils indeed originate from insoluble residues of bedrock carbonate rock.