Four strains of Bacillus spp. B15, B110, B113 and B122 were isolated from garden soil samples, which were collected from Shandong and Jiangsu Provinces. Experiments show they all had strong antagonistic effects on a broad spectrum of plant pathogenic fungi. The four strains of Bacillus spp. could induce deformed growth of hyphae of Fusarium oxysporum. With their tips bulged or attenuated, the hypha grew twisty, ramulose, yielded a large number of round cystids and their protoplasm agglomerated. Through observation of their morphological features and culture characters and a series of physiological and biochemical experiments, B15 and B113 were identified as Brevibacillus brevis, B110 as Paenibacillus polymyxa and B122 as Bacillus subtilis.