Principle, structure and characteristic of German wind-tunnel system consisting of measurement chambers, sampling unit and control unit to measure ammonia volatilization from agricultural soil were introduced in detail in this paper, and a recycling experiment was also conducted to validate it. Result showed that air pressure, temperature, humidity, wind speed inside chambers were similar to outside environment, and average ammonia recovery was over 90%, which suggested it had a qualified hermitic and homogeneous concentration-distribution environment and suitable for measuring ammonia volatilization measurements under conditions of multitreatments and repetitions in original fields, particularly for multi-factors comparison experiments. With no or less influence from weather condition, low requirement to experimental plot area, good repetition and high reliability, German wind-tunnel system can measure ammonia volatilization not only from agricultural soils, but also from storage and application of organic fertilizers as well as from various fertilizer forms.