Abstract:Sulfur deficiency has become increasingly widespread in the world. Plant and soil S analysis contributes to achievement of high production goals. A review is presented hereby on recent progress in the study on S diagnonsis technologies, such as evaluation by visible symptoms, plant tissue analysis and soil analysis. The technology of plant tissue analysis includes determination of total S, SO42--S, N/S ratio, malate/sulphate ratio, S concentration in seeds, and colorimetric method (by soaking wheat grains in glutaraldehyde). Seed S analysis is a simple and reliable method for diagnosis of S nutrition of soybean and tends to be an important method to diagnose S nutrition in plants because it can be conducted before the seeds are sown. When soil analysis is used to diagnose S status of soils, S contents in deep soil layers, sulfate leaching indexes, environmental conditions, and management history of the fields should be taken into account.