Agriculture long-term experiment is an important tool to research the long-term ecological processes, its environmental impact and regulation methods. The research progress and key functions of the international important agriculture long-term experiments were reviewed firstly, then the research status in China was analyzed of the two national network of agriculture long-term experiments, finally the suggestions to strengthen the long-term research capacity were putted forward. The progress showed that the long-term experiments enlarged their research fields on ecology and environment, which became the base for multi-discipline research and application research. Two domestic agriculture research networks mainly studied the water and nutrient cycling in agro-ecosystems. It is need to reinforce the multi-discipline research on agriculture, ecology and environment science; enlarge the experiment plot area and supplement treatments; perfect the analysis methods, and build the date and sample storage system. Finally the national policy to manage and financially support the agriculture long-term research should be designed and conducted.