Fungi plays an important role in some of soil biochemical processes such as ammoniation, nitrification, nitrogen transformation, cellulose and humus decomposition. Studies on fungi diversity and its distribution in different-size aggregates can help to understand these processes. Effect of ridge-no-tillage on the distribution of soil fungi in different-size soil aggregates were studied. The results indicated that the amount of fungi was the most within the soil aggregates of 1.0~0.25 mm under ridge-no-tillage, and the diversity of fungi showed similar trends. The amount of fungi were the most within the soil aggregate less than 0.053 mm under rice/rape rotation system. The results indicated that ridge-no-tillage may provide a more favorite micro-environment for microorganisms.