Fertilization and variety are the main factors limiting soybean yield in red soil area. To explore the effects of the interaction between fertilization level and variety on soybean yield and plant nutrient accumulation, A dual factor completely randomized block design was adopted in this study , three fertilization levels were respectively set for the main treatment, K (no fertilization), L1 (formula fertilizer 1), and L2 (formula fertilizer 2), and four soybean varieties were respectively set as vice treatment, CK (Zhongdou 41), A (Nannong 99-6), B (Nannong 57), and C (Nannong 66). Effects of 12 treatments on soybean grain and straw yield, yield increase rate, soil nutrients, and plant nutrient accumulation were analyzed. The results indicate that, there was significantly increase soybean grain and straw production under fertilization level and variety interaction(P<0.05). Compared with K treatment, L1 and L2 treatments increased soybean grain yield by an average of 27.06% and 38.01%, respectively. And compared with CK vice treatment, yield of soybean B and C increased by 19.88% and 33.88%, respectively. There is no significant difference in soybean nitrogen production ability, while there is a slight difference in phosphorus and potassium production ability under fertilization level and variety interaction. Compared with K treatment, different soybean varieties showed a trend of increasing yield under L1 and L2 treatments, while The average yield increase of L2 treatment was 9.77% higher than that of L1 treatment, and the soybean varieties showed B>CK>C>A. Fertilization level and variety interaction can significantly increase the content of soil available nitrogen, available potassium, and available phosphorus. Compared with L1, soil alkaline nitrogen of 2 treatment increased by an average of 30.48 mg·kg-1，available phosphorus and available k decreased by 2.31 mg·kg-1 and 0.62mg·kg-1, but alkali hydrolyzed nitrogen, available phosphorus, and available potassium of B variety were all increased. There was significantly increase the accumulation of nitrogen, phosphorus, and potassium nutrients in soybean plants under fertilization level and variety. And it can be seen that, relationship between soil nutrients during the flowering and podding stages and nutrient absorption by soybean plants during the mature stage, there is significantly positively correlated between? content of soil alkaline hydrolyzed nitrogen, available phosphorus, and available potassium with absorption of nitrogen, phosphorus, and potassium nutrients of plants. Thus, fertilization level of main treatment L2 and soybean variety secondary treatment B is beneficial for soybean yield and plant nitrogen, phosphorus, and potassium accumulation under fertilization levels and soybean varieties interaction, which can be used as a recommended fertilization level formula for soybeans and for promoting suitable soybean varieties.