Phosphobacterium GJT-1 was isolated from the rhizosphere of crop and was identified as Pseudomonas sp., based on its physiological characteristics and 16S rDNA sequence analysis. Phosphate-dissolving activity was measured in solid media as well as in liquid media using different phosphate sources including tricalium phosphate, aluminium phosphate, ferric phosphate. When phosphobacterium GJT-1 was inoculated into NBRIP liquid culture, the release of maximal available soluble phosphorus reached to 224.51 mg/L after 3 days incubation at 28℃. The strain showed high phosphate-dissolving activity for rock phosphates from Kaiyang in Guizhou Province and Yichang in Hubei Province with the capacity of 194.25 mg and 120.59 mg soluble phosphate respectively per liter medium. SEM study of the phosphorite powders retrieved from the phosphobacterium GJT-1 cultured medium revealed the actual dissolution of phosphate from the mineral surface.