An experiment was conducted to study the effects of tillage practices on soil fauna in areas of two crops a year of wheat-corn rotation in Huang-Huai-Hai Plain. Five treatments included: corn tillage annually + wheat tillage annually (CTWT), corn no-tillage annually + wheat tillage annually (CNTWT), corn no-tillage annually + wheat tillage every other year (CNTW2T), corn no-tillage annually + wheat tillage every four years (CNTW4T), corn no-tillage annually + wheat no-tillage annually (CNTWNT ). The results showed that: mesofauna and microfauna together accounted of 83% - 91% of total soil fauna, and macrofauna accounted for 9% - 17%. More soil fauna distributed at 0 – 10 cm soil layer which accounted of 71.9% - 73.2% of total soil fauna. Significant seasonal dynamics was observed for soil fauna, the richness of soil fauna was higher in corn season than in wheat season. The evenness of soil fauna was higher in tillage plots than in no-tillage plots in corn season but Shannon diversity index was contrary. By redundancy analysis, it suggests that tillage practices primarily influence seasonal dynamics and vertical distribution pattern of soil fauna, thus cause the changes in richness and diversity of soil fauna.