Study was conducted on the aging process of phenanthrene(PHE) and pyrene (PYE) under single and co-contaminant conditions. Results indicated that the extractable PHE and PYE decreased as their contact time with soil increased during 150 days. The aging rates of PHE and PYE in soil were rather high at the initial stage, and then slowed down with time, besides, the decreasing trend of extractability by using hydroxypropyl-β-cyclodextrin (HPCD) extraction was more obvious than using soxhlet extraction as time passed. The extractability of PYE by soxhlet extraction in co-contaminated soil was a bit lower than that in single contaminated soil in aging process. At the later stage of aging, HPCD extractability of PHE in co-contaminated soil was slightly higher than that in single contaminated soil. Earthworms were used as test organisms to reveal the aging effect on the bioavailability of two pollutants, and it was found that earthworm uptake and bioconcentration（BCF) of pollutants decreased in aging process. The PHE in co-contaminated soil was more available than that in single contaminated soil during aging. Correlation analyses showed that earthworm uptake of PHE and PYE in single contaminated soil were significantly correlated with their extractability in HPCD extraction.