To find environment-friendly fertilization modes which can increase crop yield and quality, a field experiment with spring maize was conducted to study the effects of 7 different fertilization modes on the cob’s length, yield, nitrogen accumulation, fertilizer nitrogen utilization of corn and the loss of TN, NH4+-N, NO3--N from corn field under natural rainfall condition in Dongting Lake region. The results showed that: The two kinds of fertilization modes, namely, 20% of the Biogas and 30% of pig-manure compost, improved agronomic properties of spring maize, increased yield by 69% and 73%, increased protein content by 42% and 36%, increased nitrogen accumulation by 125% and 117% respectively compared with no fertilization; decreased the surface runoff of TN by 25.1% and 38.3% respectively compared with the chemical fertilization mode. Thus these two modes could promote utilization rate of the nitrogen fertilizer and reduce the risk of agricultural non-point pollution, and should be widely used in spring maize production in Dongting Lake region.