Na, Fe, Mn, Cu and Zn contents in soil samples in 0-10, 10-20 and 20-30 cm layers in Subei alpine steppe were studied under four kinds of grazing intensities, i.e., no grazing, light grazing, middle grazing and high grazing. The results indicated that: 1) grazing intensity had consistent effect on soil physical, chemical and biological fertilities, which were associated with each other and determined the evolution direction of soil fertility; 2) With the increase of steppe grazing intensity, soil bulk density at both 0-10 cm and 10-20 cm layers increased, while soil porosity and moisture content decreased significantly, steppe grazing intensity influenced soil moisture content more strongly than soil porosity; 3) Soil organic matter and total N contents of light grazing steppes were higher than those of middle grazing and high grazing steppes, soil available N, P and K contents increased with the increase of steppe standard of grazing intensity; 4) The nutrient content order was Na＞Fe＞Mn＞Cu＞Zn, they shared the same changing order, i.e., light grazing＞no grazing＞middle grazing＞high grazing; 5) With the increase of steppe grazing intensity, the decreasing extent of soil nutrients was 10-20cm＞0-10cm＞20-30cm.