To improve the water solubility of β-cyclodextrin, β-cyclodextrin reacted with glycine in the presence of KOH and epichlorohydrin in water, a high water soluble glycine-β-cyclodextrin was prepared. Glycine-β-cyclodextrin for enhancing the solubilization and desorption behavior of anthrancene in soil were studied. The influence factors on desorption of anthrancene, such as pH, initial concentration of glycine-β-cyclodextrin, temperature and CD type were also investigated and described in details. The results showed that glycine-β-cyclodextrin has obvious solubilization for anthrancene, the solubility of anthrancene in 50 g/L of glycine-β-cyclodextrin was enhanced about 20-fold, the desorption efficiency of glycine-β-cyclodextrin for anthrancene in soil decreases with increasing pH, high temperature and initial concentration of glycine-β-cyclodextrin were beneficial to the desorption of anthrancene in soil, the desorption efficiency of glycine-β-cyclodextrin for anthrancene in soil was higher than those of α-cyclodextrin and β-cyclodextrin for anthrancene in soil and the desorption of glycine-β-cyclodextrin for anthrancene in soil followed the pseudo -second-order rate equation. This static desorption research could provide some fundamental information for the remediation of anthrancene-contaminated soil.