The spatial distribution characteristics of depression storage in Loess sloping field was studied with the method of spatial autocorrelation based on Moran’s I, and firstly micro-DEM with the grid size of 0.002 m derived from tillage methods of artifical dig and artifical back hoe, has been established by ArcGIS9.3 software referring data from quantified elevation values determined by home-made laser scanner with the measured plot area of 1.34 m2. Then depression storage or volume was extracted from micro-DEM using ArcGIS9.3’ hydrology tools. The results showed that, 1) depression storage presented weakly negative spatial autocorrelation due to global Moran's I was less than or equal to 0, and also, 2）the relationship between slope and spatial pattern of depression was not clear, but, 3）it’s autocorrelation was related to the amount of depression storage. In summary, spatial distribution characteristics of depression storage in sloping field presented a size of staggered and strong spatial heterogeneity pattern.