To know the spatial distribution of soil total Se and Se speciation in agricultural products, totally 473 Lanzhou soils and 122 agricultural products were sampled and analyzed. The results showed that the average total Se concentration of the topsoil in Lanzhou was (0.179±0.066) mg/kg (n=473), which ranked in middle level of China. Soil pH value was 8.39±0.26. Soil Se varied significantly among sampling sites, and the highest averaged soil Se was found in Yuzhong Area. The bioavailable Se of soils ranged from 4.63% to15.8%, averagely 8.27% of the total Se. A significantly positive correlation between bioavilable Se and toal Se in soils was observed. For the agricultural products, the total Se in rose (Rosa rugosa), Chinese wolfberry (Lycium barbarum), broccoli (Brassica oleracea var.italica) and celery (Apium graveolens) exceeded 0.015 mg/kg (a standard value for Se-enriched products in China). The organic Se covered averagely 73.4% of the total Se in these agricultural products. Generally, the areas with higher soil Se in Lanzhou could grow higher Se products. Because of alkaline properties of Lanzhou soil, it has a significant advantage in developing Se biofortification ?agricultural products.