The chemical composition and properties of wormcast have gradually become into one of the research focuses. In this paper the chemical composition and properties of two earthworm biotypes (Eisenia foetida and Metaphire guillelmi) were studied under laboratory conditions. The results were as follows: 1) significant increases (P < 0.05) in pH values and electrical conductivities were found in wormcast than that in the control soil (CK); 2) carbohydrate contents of wormcast were 31.36 - 33.81mg/kg higher than that of CK while polyphenol contents of wormcast were 4.28 - 5.08 mg/kg lower than that of CK; 3) contents of acidolysis amino acids, plant hormone GA3s and IAAs were 3.84 - 5.06 mg/g, 35 - 114% and 78% - 236% higher than those of CK respectively; in the same environment, the two earthworm biotypes under the same biomass showed no significant differences in pH values, carbohydrate and polyphenol contents, but the effects of Eisenia foetida on electrical conductivity, phytohormone and amino acid contents were significantly higher than those of Metaphire guillelmi.