Bacillus subtilis secret different proteolytic enzymes into culture media during stationary phase. Two major proteases are Subtilisin (Apr) and Metelloproteinase (Npr), , which account for more than 90% of total activity. Both of them are of important application on industry. In this study, , these two proteases induced by different media components, , soybean cake and wheat bran were observed. After B. subtilis BY25 fermentation, , enzyme property examination showed Apr was induced by soybean cake. Gelatin zymograph was then operated and different bands were revealed among enzyme solutions. However, , 35 KD Npr protein was observed only in the enzyme solution fermented with wheat bran.