Pot experiment was conducted in paddy soils with high and low selenium(Se) contents to study Se uptake and distribution and composition of inorganic and organic Se contents of brown and milled rice of two rice cultivars (Jiefengyou629 and Gufengyou8312) that had been selected from field experiment previously. The results showed that the accumulation amounts in rice plant and Se contents and organic ones in brown and milled rice of two rice cultivars were more in high Se content soils than in low ones, which indicated that high Se content paddy soils was contributed to Se accumulation and composition of organic Se. Therefore Se-enriched rice cultivars had better plant in high Se content paddy soils to obtain high quality Se-enriched rice. Whether in high or low Se content soil, the difference of Se accumulation amounts in plant or grain between two rice cultivars was not significant. But Se content and organic Se content in brown and milled rice of Jiefengyou629 were higher than those of Gufengyou8312, which suggested that Se absorbed by Jiefengyou629 was easily distributed to edible parts (brown and milled rice) and inorganic Se of brown and milled rice was also easily changed into organic one. Therefore, the characteristics of Se uptake and distribution of Jiefengyou629 was better than those of Gufengyou8312.