The bio-venting experiment was investigated in the laboratory using diesel as a model contaminant, performed in columns filled with soils. The removal of TPH and the factors in affecting the efficiency of diesel degradation were studied. The results showed that: 1) after three month bio-venting, the removal rate of the four different columns was Ⅱ＞Ⅰ＞Ⅳ＞Ⅲ, the initial diesel concentration was 5 g/kg (ColumnⅠ), 10 g/kg (ColumnⅡ)，20 g/kg (ColumnⅢ) and 40 g/kg (ColumnⅣ), respectively. The optimal bio-venting velocity was column?Ⅱ, whose half life was 60.05 d, the removal rate of TPH was 65.3%. 2) The diesel removal in the column was influenced by volatilization and biodegradation. The downward migration impact which caused by the gravity influenced the diesel’s distribution on the space in column. The removal rule of diesel columns with different sampling port was decided by the three actions together. 3) The change of pH was very little in the process of experiment; the loss of water was less when the initial diesel concentration was higher. Available phosphorus and available nitrogen contents were reduced, but the degrees varied from each other. The reduction rate of column Ⅲ was maximum, they were 58.27% and 31.87% respectively. The columnⅠwas least. The soil enzyme activities in upper soils were less than those in lower soils, the activities of catalase and dehydrogenase increased firstly and then decreased, which provided an important basis for the research of the diesel biodegradation in each column.