Four years’ dustfall at six functional zones in Nanjing and meteorological factors, along with the human impacts were studied in this article. Results showed that the background dustfall in the weak anthropogenic-impacted site was constant and at low levels. Due to the long-range transported Asian dust, the background dustfall in spring season was two times higher than in other seasons. The difference of dustfall among functional zones was significant, lowest at background site, and highest at industrial zones. Because the anthropogenic impact was relatively weak, the temporal variation of dustfall and meteorological influence at residential zone and suburban were similar to that at background site. Dustfall were all significantly reduced by the humidity. The anthropogenic impact on dustfall at industrial and residential mixture zone and industrial zone was heavy, making the influence of meteorology was complicated, and rain was an important factor there. No clear relationship between dustfall and meteorological factors was founded at traffic belt.