An experiment was conducted at the flue-cured tobacco breeding base in Longgang Town Kaiyang County of Guizhou Province in 2010 and 2011. Three soils of Tianzhu sandy loam (TZSL), Weining silty loam (WNSL) and Longgang clay loam (LGCL) were set under the same climatic condition to study the effects of different soil types on antioxidant systems in middle and upper leaves in the medium and later term of tobacco plant growth and development and appearance quality, sensory quality after first baking. The results showed that, the activities of PPO, SOD and POD in TZSL and WNSL were higher at the medium term of tobacco plant growth and development, the effects of LGCL became more obviously important during the latter stage of growth. The contents of MDA in middle leaves and upper leaves in TZSL and WNSL were higher than that of LGCL. The appearance quality and sensory quality of flue-cured tobacco in TZSL were best, followed by LGCL and WNSL. The time of entering ripening and senescence period in TZSL and WNSL were earlier and LGCL was later, TZSL is conducive to becoming of high-quality tobacco leaves.