The influences of biochar on the biomass of tobacco, antioxidant enzymes activity in leaves, cadmium (Cd) content in tobacco, rhizosphere soil pH and soil available Cd content under the organic fertilizer and compound fertilizer were studied with pot experiment to investigate the effect of combination of biochar and conventional fertilization on tobacco growth and Cd cumulation. The results showed that the combination of biochar and conventional fertilization promoted the growth of tobacco plants, increased the biomass of each part of tobacco and enhanced the root activity and antioxidant enzyme activity of tobacco. Among the five groups of experiment, the transport capacity of Cd in tobacco plant showed that stem/root＜leaf/root, Cd content in tobacco leaves were in order of lower leaves >mid-leaves > upper leaves, Cd contents in tobacco were of leaf > stem > root. The treatment with biochar was less effective than organic fertilizer in decreasing Cd content in top leaves, but more effective in decreasing Cd content in root. Cd content of various parts of tobacco was significantly decreased under the combination of biochar, organic fertilizer and compound fertilizer. The combination of organic fertilizer and compound fertilizer was more effective than the combination of biochar and compound fertilize in reducing Cd pollution in tobacco because biochar treatment significantly increased soil pH and compound fertilizer decreased soil pH. Biochar and organic fertilizer decreased the content of available Cd whose content was extremely significantly negative correlation with rhizosphere pH. As a result, the tobacco under the combination of biochar, organic fertilizer and compound fertilizer reduced Cd absorption because the combination led better growth and stronger resistance to tobacco.