In order to disclose the effects of nano-carbon synergistic fertilizer (NCSF) on agronomical characters and economic indices of flue-cured tobacco, and to assess the application prospect of NCSF in flue-cured tobacco production of southeastern China, a field plot experiment was conducted on K326 cultivar in 2014 with three treatments, i.e., experienced dosage of normal fertilizer (CK), equivalent experienced dosage of NCSF (T1) and reduced 20% experienced dosage of NCSF (T2). The tobacco agronomical character indices were surveyed during the rosette stage, fast growing stage and round top stage and the economic indices were assessed after grading the flue-cured tobacco leaves. It was showed that compared to CK, T1 could optimize the field agronomical characters, raise reasonably the single leaf weight and the yield of leaves, increase the proportions of medium and high class leaves and average price of leaves, and could increase the output value by 3 300 yuan/hm². The above results proves the good application prospect of the nano-carbon in tobacco production in southeastern China.