In order to improve the quality of bio-organic fertilizer (BIO), addition of amino acids to assist with the reproduction of the functional microbe inoculated into matured composts though solid-state fermentation is demonstrated to be an efficient way. An addition of significant quantity of oil rapeseed cakes could lead to a much better growth of microbial species added during the BIO preparation, however, the higher production costs hindered severely the large-scale extension of BIOs. This study investigated the possibility to produce bio-organic fertilizer (BIO) by using hydrolyzed feather powders as a solid-state fermentation (SSF) medium. The results showed that the biomass of functional strain increased with the increasing amount of hydrolyzed feather powders added in the matured compost with best additive amount of 50 g/kg. Pot experiments showed that the produced BIO efficiently promoted the growths of eggplant and tomato; compared with the control, the new produced BIO increased plant height, stem diameter and SPAD values by 19.02%, 29.02% and 4.80%, respectively, after transplanting for 20 days for eggplant; and by 14.74%, 18.70% and 4.74%, respectively, after transplanting for 50 days for tomato. In conclusion, supplemented with hydrolyzed feather powders has certain advantages in solid-state fermentation of the PGPR strain and can produce high quality bio-organic fertilizer for promoting plant growth. The above results provide the theoretical and technical supports for the development of BIOs.