Taking a typical organic and heavy metal contaminated site as the study object, the soils were divided into four layers, backfill soil layer (0 – 4.1 m), silty clay layer (4.1 – 6.5 m), silt soil layer (6.5 – 8.5 m) and silty sand layer (8.5 – 13.8 m) based on hydro-geological characteristics. Layered health risk assessment was carried out by HERA software. The risk levels and remediation targets of concerned contaminants in soil and groundwater were estimated, and on the basis remediation area and technology were determined. The results indicated the presence of serious organic (benzene and methylbenzene) and heavy metal (Cr(Ⅵ)) contamination in soil. The maximum carcinogenic risk of benzene and non-carcinogenic hazard quotient of methylbenzene were 0.000 155 and 2.14; Cr(Ⅵ) in surface soil had carcinogenic risks (0.014 2) and non-carcinogenic hazard quotients (97.6), while Cr(Ⅵ) in lower soil was not harmful to health due to no exposure pathway. The health risk of concerned contaminants in groundwater was within the acceptable range. The remediation targets of benzene and methylbenzene in different layers were 0.434, 708 mg/kg in backfill soil layer, 0.807, 2 460 mg/kg in silty clay layer, 1.42, 4 440 mg/kg in silt soil layer and 2.51, 8 140 mg/kg in silty sand layer. However, the remediation target of Cr(Ⅵ) only calculated was 0.251 mg/kg in backfill soil layer. The remediation targets of VOCs (benzene and methylbenzene) increased with soil depth increase, while the remediation targets of heavy metal (Cr(Ⅵ)) did not follow this rule. Therefore, layered health risk assessment is more suitable for the health risk assessment of VOCs.