The effects of the application of vermicompost mixed with two different functional probioticsBacillus amyloliquefaciens (X) and Fluorescent pseudomonas (Y) on the fertility and microbial properties of soil were studied under the same nutrient level. The results showed that whether mixed with probiotics or not, compared with chemical fertilizer and conventional compost, vermicompost could improve soil available nutrient contents，increase soil microbial biomass and enzyme activities significantly. In terms of soil NO₃^– content, the application of vermicompost was 14.7% higher than conventional compost. The application of probiotics promoted the activation of soil nutrients, increased the number of soil bacteria, actinomycetes, bacillus amyloliquefaciens and fluorescent pseudomonas, decreased the number of soil fungi, and improved soil enzyme activities significantly. The application of vermicompost mixed with probiotics was more obvious in increasing soil available nutrient contents, soil microbial biomass carbon and nitrogen, the amounts of soil microbial biomass and enzyme activities than other treatments, and soil bacteria number and invertase of vermicompost mixed with probiotics XY were 32.0% and 14.4% higher than conventional compost mixed with probiotics XY. Variance analysis showed that significant interactions between organic compost and probiotics on soil available phosphorus, available potassium, microbial biomass carbon, bacteria and fungi numbers, urease and invertase activities. These results indicated that the vermicompost mixed with probiotics has significant improving effects on soil fertility and microbial properties, which is a good choice of the replacement of chemical fertilizers for protecting vegetable green production and soil fertilities improvement.