Six kinds of substrates under different formulas of Chinese traditional medicine residues, composed mushroom residue, peat coal, vermiculite, pearlstone and slow-release fertilizer (N₂O︰P₂O₅︰K₂O=15︰15︰15) were prepared, and their effects on the seedling growth of tomato were studied with the imported peat substrate as the comparison (CK). The results showed that the tomato grew better under the 5 th substrate of 5 medicine residue︰1 peat︰1mushroom residue︰1.5 vermiculite︰2 perlite and 2 kg/m³ slow-release fertilizer. The principal component analysis (PCA) and cluster analysis also showed that this 5 th substrate was the best formula, which could be recommended as the seedling substrate of tomato.