Bio-organic fertilizer is a new type of fertilizer which combines the advantages of beneficial microorganism and organic fertilizer, thus can promote the colonization and development of beneficial microorganism in soil. A field experiment was conducted to disclose the effects of different bio-organic fertilizers on potato production and soil biochemical characteristics in order to overcome the obstacle of potato continuous cropping. In this study, the emergence rates, agronomic traits, yields and qualities of Weiyu 5, a potato variety, as well as soil biological properties were compared under the same amounts of chemical fertilizer and of different bio-organic fertilizers. The results showed that bio-fertilizers improved potato production and soil nutrients and microbial community. Compared with CK (only chemical fertilizers), GZ-I treatment was the best in increasing potato yield, increased by 52.26%, and also increased the ration of bacteria to fungus (B/F) by 5.67 times. GZ-III was the best in improving potato quality, which had the highest contents of starch, reducing sugar and Vc, increased by 0.68, 1.64 and 0.41 times, respectively. Bio-fertilizers improved soil nutrient contents and soil fertility, in which GZ-III was best. In general, bio-organic fertilizer can improve soil quality and increase the yield and quality of potato under continuous cropping, GZ-I is best in promoting yield and CZ-III is best in promoting soil and potato qualities.