In this study, the boron (B), molybdenum (Mo), and iron (Fe) were compounded into single and compound foliar fertilizers, and applied to strawberry seedling growth, then the optimum application amounts of B, Mo and Fe fertilizers for the growth of strawberry were determined. The results showed that the single application of B, Mo, Fe, and compound foliar fertilizers could promote the number of strawberry breeding seedlings, maternal plant height, the net photosynthetic rate, leaf area, SPAD value, aboveground and underground biomass of maternal and child seedling in different degrees. The positive effects of B and Mo on propagation of seedling number and leaf areas, and the positive effects of Fe on chlorophyll and photosynthesis were obvious. Overall, with the increase of Fe concentration, the promoting effects on the maternal plant leaf area, SPAD value, maternal plant aboveground and belowground biomass and leaf area of the seedlings were more obvious. By contrast, the promoting effect of high concentration of B and Mo was weakened, and even inhibited the number of breeding seedlings. In different trace element fertilizers, B and Mo had the best effect, significantly improving the strawberry maternal and child seedling shoot and root biomass. In summary, the treatment of 0.2% B fertilizer, 0.2% Mo fertilizer, all of the concentration of Fe fertilizer, and B and Mo compound fertilizers were optimal for strawberry seedlings.