In order to evaluate the fusion effects after and before soil being removed from long-term located experiment, one removed soil column was chosen to study the differences of chemical and physical properties between soils in commissure and in different distances from the commissure of soil column. The results showed that the field water capacity of 0-20 cm layer at 50 cm far from the commissure in the soil column was 5% higher but bulk density was 4% lower than those in commissure, respectively, and the variation coefficients were also higher than those in other layers. There was no significant difference between soils in the commissure and in other places in 20-40 cm layer, however, soil solid rate and bulk density were higher while field water capacity was lower than other layers. In 80-100 cm layer, soil solid rate and bulk density in the commissure were 4.3% and 8.3% lower while the gas rate was 7.6% higher than those in other places. The porosity in 0-40 cm layer was lower than 40-100 cm layers, with maximal porosity in 80-100 cm layer and minimal porosity in 20-40 cm layer. Soil saturated hydraulic conductivity was 35.3-38.0 cm/d in 0-20 cm layer, and decreased with the increase of depth, lower than 20 cm/d in other layers, but soil saturated hydraulic conductivity in the commissure in 80-100 cm layer was 4 times higher than that of topsoil. The rapid available nutrients in the same layers had no significant differences in different places. Total nitrogen in the commissure were significantly higher than those in other places (P<0.05). pH was increased but available nitrogen and soil organic carbon were reduced with the increase of depth. The segmented and removed soil in the commissure is still loose in the deep layers even after 5 years, and soil fusion effect is decreased with the increase of depth.