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刘国明,万梦雪,贾萌萌,王慧峰,胡文友,黄 标.异噻唑啉酮对土壤微生物杀灭效果及其影响因素初探[J].土壤,2018,50(1):101-108. LIU Guoming,WAN Mengxue,JIA Mengmeng,WANG Huifeng,HU Wenyou,HUANG Biao.Effectiveness and Influence Factors of Isothiazolinones in Soil Sterilization[J].Soils,2018,50(1):101-108 本文二维码信息
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异噻唑啉酮对土壤微生物杀灭效果及其影响因素初探
Effectiveness and Influence Factors of Isothiazolinones in Soil Sterilization
投稿时间:2016-12-15  修订日期:2017-03-20
DOI:10.13758/j.cnki.tr.2018.01.014
中文关键词:  异噻唑啉酮  卡松CG  土壤微生物  土壤灭菌
Key Words:Isothiazolinones  Kathon CG  Soil microbes  Soil sterilization
基金项目:国家自然科学基金项目(41473073)资助。
作者单位E-mail
刘国明 中国科学院土壤环境与污染修复重点实验室(南京土壤研究所) gmliu15@issas.ac.cn 
万梦雪 中国科学院土壤环境与污染修复重点实验室(南京土壤研究所)  
贾萌萌 中国科学院土壤环境与污染修复重点实验室(南京土壤研究所)  
王慧峰 中国科学院土壤环境与污染修复重点实验室(南京土壤研究所)  
胡文友 中国科学院土壤环境与污染修复重点实验室(南京土壤研究所)  
黄 标 中国科学院土壤环境与污染修复重点实验室(南京土壤研究所) bhuang@issas.ac.cn 
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中文摘要:
      异噻唑啉酮杀菌剂是一类新型的高效杀菌剂,广泛应用于个人洗护用品、制药、水处理等领域,但是目前其对土壤微生物的杀灭效果和影响因素尚不明确。本文将异噻唑啉酮杀菌剂卡松(Kathon)CG用于土壤灭菌,采用稀释平板计数法测定了供试土壤加入卡松CG前后细菌、真菌和放线菌的数量变化,以评估卡松CG对土壤微生物杀灭效果,并考察了卡松CG浓度、pH、温度、时间等对杀灭效果的影响。结果表明:卡松CG对两种土壤中放线菌杀灭率均为100%,对细菌、真菌的杀灭率随卡松CG浓度的升高而增大,当卡松CG浓度由0.5 ml/L 升至3.0 ml/L时,对风干土壤中细菌、真菌的杀灭率分别由84.1%、78.0% 升至98.7%、99.6%,卡松CG浓度升至5.0 ml/L 时,对细菌和真菌杀灭率接近100%;当卡松CG浓度较低(<3.0 ml/L)时对新鲜土壤中细菌和真菌杀灭率与风干土壤相比有所下降,此时过高的pH也会降低细菌和真菌的杀灭率,但当卡松CG浓度>3.0 ml/L时,对风干土壤和新鲜土壤中细菌、真菌杀灭率均>99.0%,pH对杀灭率影响较小;土壤温度(25 ~ 50℃)对土壤细菌杀灭效果没有影响,且卡松CG对土壤细菌杀灭作用持续时效>72 h。本研究表明,卡松CG可以在较低浓度下有效杀灭土壤中细菌、真菌和放线菌,杀灭效果在25 ~ 50℃内保持稳定,受土壤pH影响较小。采用卡松CG可以作为土壤研究中控制生物因素的备选方法之一。
Abstract:
      Isothiazolinones are a group of effective biocides, which are widely used in personal hygiene, pharmaceutical and water treatment products, but their application effects for soil sterilization are still unclear. In this paper, a commercially sold isothiazolinone biocides Kathon CG was tested for soil sterilization. The populations of bacteria, fungi and actinomycetes in tested soil were determined before and after isothiazolinone application at different concentrations, soil suspension pH values and temperatures for two durations of equilibration time. The results showed that the killing rates of actinomycetes in the tested soils were 100%, and the killing rates of bacteria and fungi in the tested soils increased with the increase of Kathon CG concentration. When Kathon CG concentration enhanced from 0.5 ml/L to 3.0 ml/L, the killing rate of bacteria and fungi in air-dried soil increased from 84.1% and 78.0% to 98.7% and 99.6%, respectively, the killing rate of bacteria and fungi was close to 100% when Kathon CG concentration reached to 5.0 ml/L or above. When the concentration of Kathon CG was 0.5 ml/L and 1.0 ml/L, the killing rates of bacteria and fungi for fresh soil declined compared that of the air-dried soil, and in this case, excessive alkaline soil suspension pH also reduced the killing rate of bacteria and fungi. When Kathon CG concentration reached to 3.0 ml/L or above, the killing rate of bacteria and fungi in air-dried soil and fresh soil was over 99.0%, and soil suspension pH had little effect on the killing rate of bacteria and fungi. The ambient temperature (25-50℃) had no influence on the germicidal effect of soil bacferia, and the duration of the bactericidal effect was longer than 72 h. This study shows that Kathon CG can effectively kill bacteria, fungi and actinomycetes in the soil at a low concentration. The germicidal effect remains stable at 25℃-50℃, less affected by pH of soil suspension. The use of Kathon CG may be one of the alternative methods in inhibiting soil microbial processes.
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