设为首页  |   加入收藏
王玉书,刘 海,黄建国.Cd2+ 对无机磷细菌生长及溶磷作用的影响[J].土壤,2018,50(1):211-216. WANG Yushu,LIU Hai,HUANG Jianguo.Effects of Cadmium on Growth and Phosphate Dissolving Abilities of Inorganic Phosphorus Bacteria[J].Soils,2018,50(1):211-216 本文二维码信息
二维码(扫一下试试看!)
Cd2+ 对无机磷细菌生长及溶磷作用的影响
Effects of Cadmium on Growth and Phosphate Dissolving Abilities of Inorganic Phosphorus Bacteria
投稿时间:2016-12-20  修订日期:2017-02-20
DOI:10.13758/j.cnki.tr.2018.01.028
中文关键词:    无机磷细菌  溶磷
Key Words:Cadmium  Inorganic phosphorus bacteria  Soluble phosphorus
基金项目:贵州省烟草公司遵义市公司科研项目(201503)资助。
作者单位E-mail
王玉书 西南大学资源环境学院 969014327@qq.com 
刘 海 西南大学资源环境学院  
黄建国 西南大学资源环境学院 huang99@swu.edu.cn 
摘要点击次数: 47
全文下载次数: 65
中文摘要:
      无机磷细菌(Phosphorus solubilization bacteria, PSB)是重要的土壤有益微生物,它们将植物不能吸收的难溶性无机磷转化为有效磷,对土壤磷素供应十分重要。镉是污染土壤的常见重金属,研究Cd2+ 胁迫下PSB的生长和溶磷能力,可揭示镉污染对土壤无机磷转化供应的影响,为改善植物磷素供应提供有益信息。试验以重庆市缙云山国家森林公园毛竹林地分离的4株假单胞菌为供试菌株,比较它们的溶磷能力,研究了不同浓度Cd2+ 对PSB生长和溶磷作用的影响。结果表明:供试菌株溶磷能力PSB05>PSB09>PSB07>PSB01,溶磷指数和溶磷率最高值是最低值的2.08倍和4.14倍。摇瓶培养120 h后,培养液pH表现为PSB01 > PSB07 > PSB09 > PSB05,并与培养液中的无机磷含量(y)呈显著负相关(y= - 83.148 pH+581.96,R2=0.9052,n=25)。在固体培养时,PSB05的溶磷圈和菌落直径随培养基中Cd2+ 浓度提高而降低。在Cd2+ 浓度0.5~2.0 mg/L液体培养基中,PSB05数量较无Cd2+ 对照减少22.09% ~ 68.18%,草酸、乙酸、柠檬酸和氢离子分泌量显著下降,溶磷量降低5.27% ~ 38.45%。供试菌株的溶磷能力差异显著;高浓度Cd2+ 抑制PSB05生长、有机酸和氢离子分泌及溶磷作用,不利于土壤供给磷素。
Abstract:
      Phosphorus (P) solubilization bacteria (PSB) is a group of important beneficial microbes which influence the ability of soil P supply due to the transformation of insoluble inorganic P unavailable for plants into available state. Cadmium (Cd) is a heavy metal commonly found in pollution soils. To study the growth and P dissolving ability of PSB under Cd2+ stress could be helpful to further understand the effects of P transformation and supply in Cd polluted soils and to provide the beneficial information on the improvement of plant P nutrition. In this paper, 4 PSB strains in Pseudomonadaceae genus isolated from moso bamboo (Phyllostachys pubescens) soil in Jinyun National Forest Park in Chongqing, were used to compare their P-dissolving abilities, and to study the changes in their growth and P solubilization in the culture mediums with Cd2+ added in different concentrations. The results showed that P dissolving abilities changed in a sequence of PSB05 > PSB09 >PSB07 > PSB01. The highest P dissolving index and P dissolving rate were 2.08 times and 4.14 times than those of the lowest, respectively. After being shaken for 120 hours, pH of culture solution showed an order of PSB01 > PSB07 > PSB09 > PSB05, and a negative correlation was found between soluble inorganic P and pH value (soluble inorganic P =–83.148 pH+581.96, R2=0.9052, n=25). The colony and P-dissolving-ring diameters of PBS05 strains grown on solid culture mediums were decreased with Cd2+ concentrations increased. In liquid culture mediums with Cd2+ 0.5–2.0 mg /L, the number of PSB05 strains was reduced by 22.09%–68.18% compared with no Cd2+. Simultaneously, the efflux of oxalate, acetate, citrate and proton was inhibited by PSB05 strains, and the amount of inorganic P that dissolved from insoluble phosphates was reduced by 5.27%–38.45%. PSB strains tested in the present experiment varied significantly in P dissolving abilities. Cd2+ inhibited microbial growth, organic acid and proton efflux and P solubilization at high concentrations, which is unbeneficial to P supply for in trees in forests.
查看全文  查看/发表评论  下载PDF阅读器

您是第1105069位访问者
版权所有 © 《土壤》编辑部
本系统由北京勤云科技发展有限公司设计   京ICP备09084417号
手机扫一扫看