Animal carcasses hydrolyzed by sulfuric acid solution to create compound liquid amino acids is an effectively strategy in environment protection. However, how to efficiently utilize these liquid amino acids does cause public concern. In this study, compound liquid amino acids from animal carcasses were utilized as additives into matured composts to create novel bio-organic fertilizer (BIO) containing plant growth-promoting rhizobacteria (PGPR) Bacillus amyloliquefaciens SQR9, after that the effect on plant growth promotion of novel bio-organic fertilizers were investigated. Results showed that the optimal amendment concentration of liquid amino acids was 20%, which resulted in a maximum biomass of functional strains with the number of 2.25×10⁸ CFU/g (dry weight, the same follow) that was 3 times of the control without adding liquid amino acids. Pot experiment results showed that the effect on cucumber plant growth promotion of the novel BIO were superior to the other treatments amended with different concentrations of liquid amino acids and the control. The number of strain SQR9 in rhizosphere soil reached 2.5×10⁵ CFU/g soil. Results from pot experiments of different crops showed the novel produced BIO resulted in greater plant growth compared to the treatment amended with BIO produced by adding chemical fertilizer as additive, the treatment amended with BIO produced by adding none additive, the treatment amended with organic fertilizer and the treatment amended with organic fertilizer amended with liquid amino acids. Meanwhile, the treatments amended with the fertilizers with strain SQR9 showed better plant growth than the others, indicating that strainSQR9 exhibited plant growth promotion effect. Moreover, compared to the treatment amended with BIO produced by adding chemical fertilizer as additive, the treatment amended with novel BIO is more beneficial for the function enhancement of the strain SQR9. In the rhizosphere of different crops, significant higher number of functional microbes were observed in the treatment amended with novel BIO. To sum up, adding 20% of liquid amino acids effectively enhanced the growth of functional microbes in the organic fertilizer, and the produced novel BIO showed outstanding plant growth promotion effect.