Continuous cropping obstacle is very serious in the banana plantation with basalt laterite in Hainan, reasonable rotation is one of the most effective methods to alleviate this problem. In this study, 10 indexes including pH, organic matter, available N, P and K, populations of bacteria, fungi, actinomycetes, nematode and pathogenic bacteria of banana wilt were selected to assess basalt laterite quality in banana orchards under 5 rotation modes by PCA method. The results showed that integrated quality index could actually indicate soil quality and the output and disease status of banana orchards, thus is an ideal indicator for soil quality assessment. Crop rotation could effectively improve soil quality, reduce wilt incidence and increase banana yield. Pineapple, sugar cane, chili and white gourd are suitably rotated with banana.