By using the methods of field survey and sampling as well as laboratory analysis method, this paper analyzed the contents of soil rapidly available phosphorus (RAP) and available zinc (AZ) in the red soils of the mid-altitude area in Qujing city of Yunnan Province and their interaction on phosphorous (P) and zinc (Zn) contents as well as the quality of tobacco leaves. The results showed that: 1) RAP content was generally at the low level, ranged from 0.96 mg/kg to 101.28 mg/kg, with an average of 31.34 mg/kg, RAP contents of 35.80% of the soil samples were in the range (20 ~ 40 mg/kg) suitable for tobacco growth; AZ content was abundant, ranged from 0.20 mg/kg to 23.68 mg/kg, with the mean of 3.01 mg/kg, AZ contents of 74.69% of the soil samples were in the range (1 ~ 4 mg/kg) suitable for tobacco growth; 2)P content in tobacco leaves was in the middle and low levels, with the mean of 1.9 g/kg, P contents of 95.22% of tobacco samples were in the general range (1.5 ~ 6.0 g/kg) in China; Zn content in tobacco leaves was high, with the mean of 46.12 mg/kg, Zn contents of 94.24% of tobacco samples were in the general range (20 ~ 80 mg/kg) in China; 3)P content in tobacco leaves changed irregularly with the changes of soil RAP and AZ contents; Zn content of tobacco leaves increased with the increase of soil AZ content but decreased with the increase of soil RAP content; 4)The interaction between soil RAP and AZ had no significant effect on P content in tobacco leaves but a significant effect on Zn content in tobacco leaves, and the high existence of RAP could inhibit tobacco absorbed soil AZ; 5)Soil RAP had little effect on the quality of tobacco leaves, while there was a significant correlation between soil AZ and the chemical components as well as aroma substances of tobacco leaves. Generally, tobacco leaf has a high quality when soil AZ content is ranged in the middle-low level and soil RAP content is ranged in the middle-suitable level.