A field experiment was conducted to characterize zinc distribution in rice during the tilling and the flowering stages under a high level of zinc fertilization. The results showed that zinc contents in rice tissues were positively correlated with the amount of zinc fertilization. Compared with CK, zinc contents in stems, leaves, and spikes reached up to 666.7, 461.6 and 185.5 mg/kg, increased by 27.4, 19.6 and 8.7 times, respectively; zinc contents increased by up to 28.7% and 142.6% in unpolished rice after zinc fertilization during the tilling and the flowering stages, respectively. A conducted duplicate test confirmed that it is better and more reliable to increase zinc content in rice by zinc fertilization during the flowering stage. By consuming the biofortified rice, daily intake of zinc by Chinese citizens can reach up to 12.5 mg standard recommended by Chinese Nutrition Society. This study provided theoretical and practical references to fortify zinc in rice grain via zinc fertilization and then improved the deficiency of daily zinc intake.