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张 亮,盛 浩,袁 红,赵兰凤,李华兴.荧光假单胞菌诱导番茄抗枯萎病的ISR研究[J].土壤,2018,50(5):1056-1060. ZHANG Liang,SHEN Hao,YUAN Hong,ZHAO Lanfeng,LI Huaxing.Induction of Systemic Resistance and Signal Passway Regulation by Pseudomonas fluorecens PEF-5#18 Against Fusarium Wilt Disease on Tomato[J].Soils,2018,50(5):1056-1060 本文二维码信息
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荧光假单胞菌诱导番茄抗枯萎病的ISR研究
Induction of Systemic Resistance and Signal Passway Regulation by Pseudomonas fluorecens PEF-5#18 Against Fusarium Wilt Disease on Tomato
投稿时间:2017-07-11  修订日期:2017-08-16
DOI:10.13758/j.cnki.tr.2018.05.027
中文关键词:  荧光假单胞菌  诱导系统性抗性  枯萎病  信号传导路径
Key Words:Pseudomonas fluorecens  Induced systemic resistance  Tomato wilt  Signal passway
基金项目:广东省教育部产学研结合项目(2009B090300330)资助。
作者单位E-mail
张 亮 湖南农业大学资源环境学院 qingzhiweiwu@sina.com 
盛 浩 湖南农业大学资源环境学院  
袁 红 湖南农业大学资源环境学院  
赵兰凤 华南农业大学资源环境学院  
李华兴 华南农业大学资源环境学院 huaxli@scau.edu.cn 
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中文摘要:
      本文采用实时荧光定量PCR技术对荧光假单胞菌PEF-5#18诱导番茄系统性抗性(ISR)的作用机理进行了研究,包括了诱导进程中番茄根系病程蛋白基因PRs族、GR等重要防卫基因以及乙烯、水杨酸、茉莉酸等诱导信号调控路径的关键基因的表达情况。结果表明,尖孢镰刀菌Forl和荧光假单胞菌PEF-5#18对番茄植株分别具有SAR和ISR诱导抗性能力,而在“Forl-番茄-PEF-5#18”互作模式下,番茄植株受诱导所产生的抗性则受到SAR和ISR共同作用的影响;与对照相比,Forl或荧光假单胞菌PEF-5#18均能诱导番茄病情蛋白基因PRs (PR1PR4PR5PR6) 与GRPOXPAL等相关防卫基因的表达进行上调,而相关受诱导基因的表达程度与动态变化则与所诱导的SAR和ISR抗性反应类型以及诱导时间有关;此外,对于调控路径的分析结果显示出尖孢镰刀菌Forl对番茄SAR诱导抗性主要依赖于水杨酸路径进行调控,而荧光假单胞菌PEF-5#18对番茄ISR诱导抗性则主要依赖于茉莉酸和乙烯路径来调控。
Abstract:
      In this study, the expressions of pathogenic protein genes (PRs), defence related genes like GR, etc., and signal regulated key genes of SA, JA, ET passway were analyzed through RT-PCR technology in order to understand the mechanism of induced systemic resistance by P. fluorescens PEF-5#18 on tomato. The results showed that the pathogenic Forl and P. fluorescens PEF-5#18 could induce tomato plant resistance by SAR or ISR, respectively, whereas, under the interaction condition of Forl-Tomato- PEF-5#18, the joint-inducing effects from SAR and ISR affected tomato’s resistance. Compared with the control treatment, all of the PRs family (PR1, PR4, PR5, PR6) genes as well as the related defense genes (GR, POX, PAL) were up-regulated, however, the expressing values and dynamic changes of induced genes were influenced by the SAR or ISR type and inducing time. In addition, the results implied the SAR induced by Forl was regulated through SA singal passway, and the ISR induced by P. fluorescens PEF-5#18 was mainly depend on JA or ET singal passway.
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