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黄洁雪,闫明科,薛彩雯,沈仁芳,兰 平.外界铁浓度调控缺磷植物铁吸收相关基因的表达量[J].土壤,2018,50(5):866-873. HUANG Jiexue,YAN Mingke,XUE Caiwen,SHEN Renfang,LAN Ping.Phosphate Deficiency Induced Down-regulation of Iron Acquisition Genes is Dependent on Ambient Iron Concentrations[J].Soils,2018,50(5):866-873 本文二维码信息
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外界铁浓度调控缺磷植物铁吸收相关基因的表达量
Phosphate Deficiency Induced Down-regulation of Iron Acquisition Genes is Dependent on Ambient Iron Concentrations
投稿时间:2017-07-25  修订日期:2017-08-23
DOI:10.13758/j.cnki.tr.2018.05.002
中文关键词:  缺磷  铁吸收  磷铁互作
Key Words:Phosphate-deficiency  Iron uptake  Interaction between phosphate and iron
基金项目:国家重点研发计划项目(2016YFD0200308)、国家重点基础研究发展计划(973计划)项目(2015CB150501)和中科院南京土壤研究所135领域前沿项目(ISSASIP1605)资助。
作者单位E-mail
黄洁雪 土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所) jxhuang@issas.ac.cn 
闫明科 土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所)  
薛彩雯 土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所)  
沈仁芳 土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所)  
兰 平 土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所) plan@issas.ac.cn 
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中文摘要:
      磷和铁都是植物必需营养元素,缺磷和缺铁都会严重影响植物生长发育导致作物产量和品质下降。前期研究表明缺磷会导致植物铁吸收基因的表达量下降,但这种下降与外界铁浓度是否相关还不清楚。本文检测了缺磷和正常磷条件下不同铁浓度对植物铁吸收基因的表达变化。结果显示,缺磷导致植物主根生长受到显著抑制,但该抑制现象和铁浓度显著相关,在铁浓度下降到一定范围后该抑制作用消失。qPCR结果显示,缺磷显著诱导缺磷响应基因IPSSPX3PHT1;4表达量增加,且这种表达量增加仅受缺磷诱导,和铁浓度无关。缺磷也显著诱导铁吸收相关基因FRO2IRT1CYP82C4的表达量下降,但这种下降具有明显铁浓度依赖性。随着铁浓度升高,缺磷诱导的铁吸收基因的表达量下降幅度随之增大,这可能是由于缺磷导致培养基中铁的有效性增加所致。本研究结果为土壤磷、铁肥料管理提供了新的视角。
Abstract:
      Phosphorus (P) and iron (Fe) are two essential nutrients for plants, and their low availability in soil would seriously constrain crop growth and development, leading to compromised yield and quality. The down-regulation of genes involved in Fe uptake in phosphate (Pi)-deficiency were reported previously, but it is unclear whether there is a dependency on Fe concentration contributing to the disturbance. In this research, the variation of the transcript abundance of Fe acquisition genes upon Pi-deficiency with different concentrations of Fe was analyzed. Results showed that the pronounced inhibition of primary root growth under Pi-deficiency was significantly correlated to Fe concentrations, and the inhibition disappeared when Fe concentrations were decreased to a certain range. qRT-PCR analysis showed that the expression levels of Pi-deficiency marker genes IPS, SPX3, PHT1;4 were dramatically and exclusively induced by P-deficiency, which is independent of Fe concentrations. The expression levels of Fe-deficiency marker genes FRO2, IRT1 and CYP82C4 were significantly down-regulated by Pi-deficiency in a Fe-concentration-dependent manner, and decreased with increasing Fe concentrations, which might due to the elevated Fe availability in Pi-deficiency media. The results proposed a new perspective for management of P and Fe fertilizers in soil.
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