An experiment was conducted to study the interaction effects of biochemical inhibitor combinations (N-(n-butyl) thiophosphoric triamide (NBPT), N-(n-propyl) thiophosphoric triamide (NPPT) and 2-chloro-6-(trichloromethyl) pyridine (CP)) and fertilization models (one-off and three-time fertilization) on nitrogen (N) dynamic change characteristics in the surface water and leachate from yellow clayey paddy field using two factor randomized block design. The results showed that N losses of surface water and leachate from yellow clayey paddy field were given priority to NH₄⁺-N and NO₃^--N, respectively. The peak values of NH₄⁺-N and TN concentrations in the surface water from paddy field appeared within one day after basal application, and then decreased by 57.9% - 69.1% and 41.9% - 59.0% (one-off), 29.9% - 60.7% and 60.9% - 69.7% (three-time) till the 6th day, respectively. The peak values of NO₃^--N and TN concentrations in the leachates from paddy field appeared within 1 - 3 days after basal application, and then decreased by 51.4% - 56.5% and 56.6% - 61.6% (one-off), 45.3% - 57.5% and 51.1% - 59.6% (three-time) till the 6th day, respectively. Under different fertilization modes, CP increased NH₄⁺-N concentration of surface water, and NBPT/NPPT or combined with CP effectively inhibited the activity of urease, and reduced the peak value of NH₄⁺-N in the surface water. On the other hand, CP significantly decreased NO₃^--N concentration of the leachate, and CP or combined with NBPT/NPPT effectively inhibited nitrification, and reduced the peak value of NO₃^--N in the leachate. Application of a new urease inhibitor NPPT alone or combined with CP had the same effect on N dynamic change characteristics in the surface water and leachate from paddy field with NBPT. In conclusion, the integration and optimization of fertilization technique and combined inhibitors application can more effectively delay urea hydrolysis, inhibit nitrification, and reduce the runoff and leakage loss of N in yellow clayey paddy field.